PCG® I-GBS Real-time PCR Kit
PCG® I-GBS Real-time PCR Kit
Principle: Multiplex Real-time PCR using Taqman Probe
Intended use: Detect cfb gene of Group B Streptococcus (GBS agalactiae) using internal control
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Overview
PCG® I-GBS Real-time PCR Kit is designed to detect Group B Streptococcus (GBS) in vaginal-rectal swabs from pregnant women. This kit amplifies a 199 bp DNA sequence of the cfb gene and measures fluorescence increase for accurate detection. Utilizing "hot start" technology, it minimizes non-specific reactions, ensuring high specificity and maximum sensitivity up to 100 CFU/mL. The kit is compatible with various Real-time PCR instruments.
Specification
| Regulatory Status | RUO |
| Intended User | For professional use in laboratories with trained |
| Technology | Multiplex Real-time PCR using TaqMan Probes |
| Type of Analysis | Qualitative |
| Target Sequence | cfb gene of GBS |
| Analytical Specification | In silico analysis of 11 GBS variants (la, lb, lc, ll, lll, IV, V, VI, VII, VIII, ND), 100% |
| Analytical Sensitivity (LOD) | 100 CFU/ mL |
| Cross-reactivity | No cross-reactions between Group B Streptococcus and 13 other species (S. aureus, E. faecium, E. coli, H. influenzae, K. pneumoniae, A. hydrophila, A. baumannii, P. aeruginosa, E. faecalis, S. mitis, C. albicans, C. glabrata, C. tropicalis) |
| Repeatability | Coefficient of variation (CV) ≤ 5% |
| Reproducibility | Coefficient of variation (CV) ≤ 5% |
| Extraction/ Inhibition Control | Proper sampling, DNA extraction efficiency and PCR inhibition control by Internal Control (beta-Actin gene) |
| Validated Specimen | Vaginal – rectal swab |
| Validated Extraction Methods |
PCG® GBS MagBead Automated DNA Extraction Kit
PANAMAX Viral DNA/ RNA Extraction Kit
|
| Instruments |
QuantStudio™ 3 Real-Time PCR System
QuantStudio™ 5 Real-Time PCR System
QuantStudio™ 7 Real-Time PCR System
CFX96/ CFX96 Dx Real-Time PCR Detection System
|
| Required Detection Channels |
FAM: Detect GBS
HEX/ VIC: Detect Internal Control
|
| Storage | 12 months, -20ºC (±5ºC) |
Workflow
Order information
| Product name | Package | REF |
|
PCG® I-GBS Real-Time PCR Kit
|
50 tests/ kit | PR2600050 |
FAQs
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