PCG® Index Ligation Kit

Technology:
PCR-free - Index attachment without amplification.

Intended use:
Attaching Index Adaptors to DNA segments for encoding various samples, and adding necessary sequences for gene decoding using Illumina sequencing systems. Suitable for high-precision applications such as non-invasive prenatal testing (NIPT), non-invasive cancer testing, and methylation level assessment.

Overview

The cfDNA marking and quantification kit is used to attach index adaptors to DNA segments, encoding them for various samples, and simultaneously purifying these DNAs for next-generation gene sequencing processes. The non-amplification index attachment technology allows library preparation with minimized error and sequence repetition.

Indications:
Specialized for laboratories with trained personnel.

Principle:
Attach indices to DNA segments using ligase enzymes without amplification (PCR-free).
Purification using SPRI beads, without gel (gel-free).
Quantification using fluorescent dye and comparison to DNA standard curves.

Specification

Regulatory Status	IVD
Intended user	For professional use in laboratories with trained
Technology	Ligate index adapters to DNA segments using a ligase enzyme without PCR DNA purification using SPRI beads without gel Fluorescence-based DNA qualification using a DNA standard curve
Automation/ Manual Extraction	Both automation and manual
Running time (Index ligation, DNA purification and quantification)	3 hours/ 48 samples
Validated specimen	cfDNA, fragmented genomic DNA, PCR products
Input sample volume	≥ 7 ng DNA
Target insert size	150 - 350 bp
Index ligation efficiency	> 30%
Storage	Box 1: -25°C to -15°C Box 2: 2°C to 8°C
Shelf life	6 months