Sperm fragmentation is the alteration and deletion of bases in the sperm DNA structure, or the cleavage of one or both strands of the DNA present in the sperm, causing the sperm DNA to break into small fragments. With current semen analysis tests, we can only assess sperm morphology, concentration, and motility, while evaluating sperm at the molecular level is not possible. However, detecting sperm DNA fragmentation can give us an insight into the integrity of the sperm genetic material, enhancing the chance of success in implementing assisted reproductive methods.  

The course “APPLICATION OF FLOW CYTOMETRY IN THE DIAGNOSIS OF SPERM DNA FRAGMENTATION” will provide students an overview of the latest test solutions on Sperm Fragmentation testing. The course focuses on practice skills that students have access to cutting-edge equipment and practice frequently on patient specimens, thereby upgrading their skills in sperm quality assessment at the molecular level. After the course, students could fully implement this technique at work.  

• Team-based learning: encourage critical thinking, creativity, and proactive learning through group discussions, and group presentations
• On-the-job training: Students observe and practice alongside the lecturers
• Teaching equipment: A 300 m2 Lab with modern equipment from many well-known manufacturers around the world

• Comprehend the technology principles of the Flow cytometry system of Beckman Coulter Life Sciences  
• Comprehend the purpose and applications of Sperm DNA fragmentation testing  
• Comprehend the Sperm fragmentation testing procedure on Flow cytometry system of Beckman Coulter Life Sciences
• Perform Sperm fragmentation testing techniques
• Comprehend the principles and operate the equipment during the testing process
• Assess and analyze results proficiently  
• Comprehend causes and solutions to common errors during the test process 

1. Overview of Flow Cell Technology 

1.1  Basic principles of flow cells 

1.2. Introduction to the basic principles of Flow cytometry data analysis, instructions on how to use charts

2. Testing practice

2.1 The procedure for preparing specimens, chemicals, and processing specimens 

2.2 The procedure for Sperm DNA fragmentation testing 

2.3 Perform Sperm Fragmentation testing techniques on Flow cytometry system of Beckman Coulter Life Sciences

2.4 Analyze results, causes, and solutions to common errors during the process 

3. Summary 

3.1 Course summary. Q&A. Certificate awarding ceremony

• Course Code: FC - 01
• Subjects: Doctors, bachelors or engineers specialized in testing in healthcare facilities; bachelors of Biology, Biotechnology, Chemistry 
• Training time: 04 days (8 lessons/day) 
• Number of students: 12 - 16 students per class 
• Course opening: Open every month upon the number of applications 
• Registration package:

- 01 Application form of Phacogen Institute of Technology HERE

- 01 notarized copy of university or college diploma  

- 01 notarized copy of identity card or citizen identification